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Abstract Conjugation is used by bacteria to propagate antimicrobial resistance (AMR) in the environment. Central to this process are widespread conjugative F-pili that establish the connection between donor and recipient cells, thereby facilitating the spread of IncF plasmids among enteropathogenic bacteria. Here, we show that the F-pilus is highly flexible but robust at the same time, properties that increase its resistance to thermochemical and mechanical stresses. By a combination of biophysical and molecular dynamics methods, we establish that the presence of phosphatidylglycerol molecules in the F-pilus contributes to the structural stability of the polymer. Moreover, this structural stability is important for successful delivery of DNA during conjugation and facilitates rapid formation of biofilms in harsh environmental conditions. Thus, our work highlights the importance of F-pilus structural adaptations for the efficient spread of AMR genes in a bacterial population and for the formation of biofilms that protect against the action of antibiotics. 

Abstract Luminous quasars are powerful targets to investigate the role of feedback from supermassive black holes (BHs) in regulating the growth phases of BHs themselves and of their host galaxies, up to the highest redshifts. Here we investigate the cosmic evolution of the occurrence and kinematics of BH-driven outflows, as traced by broad absorption line (BAL) features, due to the C iv ionic transition. We exploit a sample of 1935 quasars at z = 2.1–6.6 with bolometric luminosity log( L bol /erg s −1 ) ≳ 46.5, drawn from the Sloan Digital Sky Survey and from the X-Shooter legacy survey of Quasars at the Reionization Epoch (XQR-30). We consider rest-frame optical bright quasars to minimize observational biases due to quasar selection criteria. We apply a homogeneous BAL-identification analysis, based on employing composite template spectra to estimate the quasar intrinsic emission. We find a BAL quasar fraction close to 20% at z ∼ 2–4, while it increases to almost 50% at z ∼ 6. The velocity and width of the BAL features also increase at z ≳ 4.5. We exclude the possibility that the redshift evolution of the BAL properties is due to differences in terms of quasar luminosity and accretion rate. These results suggest significant BH feedback occurring in the 1 Gyr old universe, likely affecting the growth of BHs and, possibly, of their host galaxies, as supported by models of early BH and galaxy evolution. 

Abstract Flagellar filaments function as the propellers of the bacterial flagellum and their supercoiling is key to motility. The outer domains on the surface of the filament are non-critical for motility in many bacteria and their structures and functions are not conserved. Here, we show the atomic cryo-electron microscopy structures for flagellar filaments from enterohemorrhagic Escherichia coli O157:H7, enteropathogenic E. coli O127:H6, Achromobacter , and Sinorhizobium meliloti , where the outer domains dimerize or tetramerize to form either a sheath or a screw-like surface. These dimers are formed by 180° rotations of half of the outer domains. The outer domain sheath (ODS) plays a role in bacterial motility by stabilizing an intermediate waveform and prolonging the tumbling of E. coli cells. Bacteria with these ODS and screw-like flagellar filaments are commonly found in soil and human intestinal environments of relatively high viscosity suggesting a role for the dimerization in these environments. 

We present measurements of black hole masses and Eddington ratios (λ_Edd) for a sample of 38 bright (M₁₄₅₀< −24.4 mag) quasars at 5.8 ≲z≲ 7.5, derived from Very Large Telescope/X–shooter near–IR spectroscopy of their broad Civand Mgiiemission lines. The black hole masses (on average,M_BH∼ 4.6 × 10⁹M_⊙) and accretion rates (0.1 ≲λ_Edd≲ 1.0) are broadly consistent with that of similarly luminous 0.3 ≲z≲ 2.3 quasars, but there is evidence for a mild increase in the Eddington ratio abovez≳ 6. Combined with deep Atacama Large Millimeter/submillimeter Array (ALMA) observations of the [CII] 158μm line from the host galaxies and VLT/MUSE investigations of the extended Lyαhalos, this study provides fundamental clues to models of the formation and growth of the first massive galaxies and black holes. Compared to local scaling relations,z≳ 5.7 black holes appear to be over-massive relative to their hosts, with accretion properties that do not change with host galaxy morphologies. Assuming that the kinematics of theT∼ 10⁴K gas, traced by the extended Lyαhalos, are dominated by the gravitational potential of the dark matter halo, we observe a similar relation between black hole mass and circular velocity as reported forz∼ 0 galaxies. These results paint a picture where the first supermassive black holes reside in massive halos atz≳ 6 and lead the first stages of galaxy formation by rapidly growing in mass with a duty cycle of order unity. The duty cycle needs to drastically drop toward lower redshifts, while the host galaxies continue forming stars at a rate of hundreds of solar masses per year, sustained by the large reservoirs of cool gas surrounding them.

 

Abstract Studies of rest-frame optical emission in quasars at z > 6 have historically been limited by the wavelengths accessible by ground-based telescopes. The James Webb Space Telescope (JWST) now offers the opportunity to probe this emission deep into the reionization epoch. We report the observations of eight quasars at z > 6.5 using the JWST/NIRCam Wide Field Slitless Spectroscopy as a part of the “A SPectroscopic survey of biased halos In the Reionization Era (ASPIRE)” program. Our JWST spectra cover the quasars’ emission between rest frame ∼4100 and 5100 Å. The profiles of these quasars’ broad H β emission lines span a full width at half maximum from 3000 to 6000 km s −1 . The H β -based virial black hole (BH) masses, ranging from 0.6 to 2.1 billion solar masses, are generally consistent with their Mg ii -based BH masses. The new measurements based on the more reliable H β tracer thus confirm the existence of a billion solar-mass BHs in the reionization epoch. In the observed [O iii ] λ λ 4960,5008 doublets of these luminous quasars, broad components are more common than narrow core components (≤ 1200 km s −1 ), and only one quasar shows stronger narrow components than broad. Two quasars exhibit significantly broad and blueshifted [O iii ] emission, thought to trace galactic-scale outflows, with median velocities of −610 and −1430 km s −1 relative to the [C ii ] 158 μ m line. All eight quasars show strong optical Fe ii emission and follow the eigenvector 1 relations defined by low-redshift quasars. The entire ASPIRE program will eventually cover 25 quasars and provide a statistical sample for the studies of the BHs and quasar spectral properties. 

Abstract We present the first results from the JWST program A SPectroscopic survey of biased halos In the Reionization Era (ASPIRE). This program represents an imaging and spectroscopic survey of 25 reionization-era quasars and their environments by utilizing the unprecedented capabilities of NIRCam Wide Field Slitless Spectroscopy (WFSS) mode. ASPIRE will deliver the largest ( ∼ 280 arcmin 2 ) galaxy redshift survey at 3–4 μ m among JWST Cycle 1 programs and provide extensive legacy values for studying the formation of the earliest supermassive black holes, the assembly of galaxies, early metal enrichment, and cosmic reionization. In this first ASPIRE paper, we report the discovery of a filamentary structure traced by the luminous quasar J0305–3150 and 10 [O iii ] emitters at z = 6.6. This structure has a 3D galaxy overdensity of δ gal = 12.6 over 637 cMpc 3 , one of the most overdense structures known in the early universe, and could eventually evolve into a massive galaxy cluster. Together with existing VLT/MUSE and ALMA observations of this field, our JWST observations reveal that J0305–3150 traces a complex environment where both UV-bright and dusty galaxies are present and indicate that the early evolution of galaxies around the quasar is not simultaneous. In addition, we discovered 31 [O iii ] emitters in this field at other redshifts, 5.3 < z < 6.7, with half of them situated at z ∼ 5.4 and 6.2. This indicates that star-forming galaxies, such as [O iii ] emitters, are generally clustered at high redshifts. These discoveries demonstrate the unparalleled redshift survey capabilities of NIRCam WFSS and the potential of the full ASPIRE survey data set. 

Bacterial type IV secretion systems (T4SSs) are a functionally diverse translocation superfamily. They consist mainly of two large subfamilies: (i) conjugation systems that mediate interbacterial DNA transfer and (ii) effector translocators that deliver effector macromolecules into prokaryotic or eukaryotic cells. A few other T4SSs export DNA or proteins to the milieu, or import exogenous DNA. The T4SSs are defined by 6 or 12 conserved “core” subunits that respectively elaborate “minimized” systems in Gram‐positive or ‐negative bacteria. However, many “expanded” T4SSs are built from “core” subunits plus numerous others that are system‐specific, which presumptively broadens functional capabilities. Recently, there has been exciting progress in defining T4SS assembly pathways and architectures using a combination of fluorescence and cryoelectron microscopy. This review will highlight advances in our knowledge of structure–function relationships for model Gram‐negative bacterial T4SSs, including “minimized” systems resembling theAgrobacteriumtumefaciensVirB/VirD4 T4SS and “expanded” systems represented by theHelicobacterpyloriCag,LegionellapneumophilaDot/Icm, and F plasmid‐encoded Tra T4SSs. Detailed studies of these model systems are generating new insights, some at atomic resolution, to long‐standing questions concerning mechanisms of substrate recruitment, T4SS channel architecture, conjugative pilus assembly, and machine adaptations contributing to T4SS functional versatility.